Western blot analysis of extracts of human colon, using HRH1 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
HRH1 / Histamine H1 Receptor Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C331459-20
Catalog Number: LS-C331459
For bulk orders or custom formulations:
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- Description
- Histamine H1 Receptor antibody LS-C331459 is an unconjugated rabbit polyclonal antibody to human Histamine H1 Receptor (HRH1). Validated for IHC and WB. Cited in 1 publication.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- H1 histamine receptor | H1 receptor | Histamine 1 receptor | Histamine H1 receptor | H1-R | H1R | Histamine H(1) receptor | Histamine receptor H1 | HisH1 | HRH1
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P35367
- NCBI GenBank
- NM_000861 | NP_000852.1
- SwissProt
- P35367
Target
- Target
- Human HRH1 / Histamine H1 Receptor
- Antigen Species
- Human
- Epitope
- Human HRH1 / Histamine H1 Receptor
- Gene Name
- HRH1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant protein of human HRH1
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Concentration
- 1.18 mg/mL
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
The allergy mediator histamine confers resistance to immunotherapy in cancer patients via activation of the macrophage histamine receptor H1. Hongzhong Li, Yi Xiao, Qin Li, Jun Yao, Xiangliang Yuan, Yuan Zhang, Xuedong Yin, Yohei Saito, Huihui Fan, Ping Li, Wen-Ling Kuo, Angela Halpin, Don L Gibbons, Hideo Yagita, Zhongming Zhao, Da Pang, Guosheng Ren, Cassian Yee, J Jack Lee, Dihua Yu. Cancer cell. 2022 January;40:36-52.e9. PubMed: 34822775 PMC: PMC8779329