Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~21.3 kDa HRAS protein using Rabbit Anti-HRAS Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HRAS Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.3 kDa. Other Band(s): 25 kDa.
HRAS / H-Ras Antibody (APC)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$488.00each
Catalog Number: LS-C773433-100
Catalog Number: LS-C773433
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- Description
- H-Ras antibody LS-C773433 is an APC-conjugated rabbit polyclonal antibody to human H-Ras (HRAS). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-H-RAS | C-has/bas p21 protein | C-ras-Ki-2 activated oncogene | C-HA-RAS1 | CTLO | H-ras | H-RASIDX | GTP- and GDP-binding peptide B | GTPase HRas | H-Ras-1 | HAMSV | HRAS | HRAS1 | K-RAS | Ha-Ras | Ha-Ras1 proto-oncoprotein | Transforming protein p21 | N-ras | p21ras | RASH1 | C-BAS/HAS | C-ha-ras | p19 H-RasIDX protein
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P01112
- NCBI GenBank
- NM_005343 | NP_005334.1
- SwissProt
- P01112
Target
- Target
- Human HRAS / H-Ras
- Antigen Species
- Human
- Epitope
- Detects ~25 kDa. Band is higher than the predicted molecular weight due to post translational modifications.
- Gene Name
- HRAS
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the C-Terminus of Human HRAS (aa. 100-189)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Allophycocyanin
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS