Western blot analysis of extracts of various cell lines, using HMOX1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
HMOX1 / HO-1 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$305.00each
Catalog Number: LS-C747172-20
Catalog Number: LS-C747172
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- HO-1 antibody LS-C747172 is an unconjugated rabbit polyclonal antibody to HO-1 (HMOX1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP and WB.
- Application
- IF, IHC, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BK286B10 | HO-1 | HSP32 | HMOX1 | Heat shock protein, 32-kD | Heme oxygenase (decycling) 1 | Heme oxygenase 1 | HO1
- Recommended Dilution: IF/ICC
- 1:50 - 1:100
- Recommended Dilution: IHC
- 1:50 - 1:100
- Recommended Dilution: IP
- 1:20 - 1:50
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P09601
- NCBI GenBank
- NM_002133 | NP_002124.1
- SwissProt
- P09601
Target
- Target
- Human HMOX1 / HO-1
- Antigen Species
- Human
- Gene Name
- HMOX1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant protein of human HMOX1
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS