HMGB1 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C:0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-HMGB1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-HMGB1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa.
HMG1 / HMGB1 Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$148.00each
Catalog Number: LS-C815337-20
Catalog Number: LS-C815337
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- Description
- HMGB1 antibody LS-C815337 is an unconjugated rabbit polyclonal antibody to HMGB1 (HMG1) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB.
- Application
- ICC, IF, IHC-P, IP, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Amphoterin | High mobility group protein B1 | High mobility group box 1 | High mobility group protein 1 | HMG1 | HMG3 | SBP-1 | High-mobility group box 1 | HMG-1 | HMGB1
- Recommended Dilution: IF/ICC
- 1:300 - 1:10000
- Recommended Dilution: IHC-P
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P09429
- NCBI GenBank
- NM_002128 | NP_002119.1
- SwissProt
- P09429
Target
- Target
- Human HMG1 / HMGB1
- Antigen Species
- Human
- Epitope
- Human HMGB1
- Gene Name
- HMGB1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- A synthetic peptide corresponding to the C-Terminus of Human HMGB1/HMG1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 20 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS