Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
HLCS / HCS Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$300.00each
Catalog Number: LS-C680605-50
Catalog Number: LS-C680605
For bulk orders or custom formulations:
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- Description
- HCS antibody LS-C680605 is an unconjugated rabbit polyclonal antibody to human HCS (HLCS). Validated for ELISA and IHC.
- Application
- ELISA, IHC, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Biotin--protein ligase | HLCS | HCS | Biotin apo-protein ligase
- UniProt Number
- P50747
- NCBI GenBank
- NM_000411 | NP_000402.3
- SwissProt
- P50747
Target
- Target
- Human HLCS / HCS
- Antigen Species
- Human
- Gene Name
- HLCS
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Human Biotin--protein ligase protein (603-714AA)
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- 0.01 M PBS