Western blot analysis of extracts of various cell lines, using HLA-DRB4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.

HLA-DRB4 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Mouse
Format:	Liquid

$281.00each

Catalog Number: LS-C496790-20

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Overview

Description: HLA-DRB4 antibody LS-C496790 is an unconjugated rabbit polyclonal antibody to HLA-DRB4 from human. It is reactive with human and mouse. Validated for WB.
Application: WB
Reactivity: Hu, Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: DR-4 | DR4 | DRB1 transplantation antigen | HLA-DR4B | Human leucocyte antigen DRB4 | MHC class2 antigen | MHC class II antigen DRB4 | MHC HLA DR-beta chain | DRB4 | HLA-DRB4
Recommended Dilution: WB: 1:1000 - 1:4000
UniProt Number: P13762
NCBI GenBank: NM_021983 | NP_068818.4
SwissProt: P13762

Target

Target: Human HLA-DRB4
Antigen Species: Human
Gene Name: HLA-DRB4

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-227 of human HLA-DRB4 (NP_068818.4). GDTQPRFLEQAKCECHFLNGTERVWNLIRYIYNQEEYARYNSDLGEYQAVTELGRPDAEYWNSQKDLLERRRAEVDTYCRYNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSMMSPLTVQWSARSESAQSK
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)