Western blot analysis of extracts of various cell lines, using HLA-DRA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

HLA-DRA Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human, Rat
Format:	Liquid

$281.00each

Catalog Number: LS-C747133-20

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Overview

Description: HLA-DRA antibody LS-C747133 is an unconjugated rabbit polyclonal antibody to HLA-DRA from human. It is reactive with human and rat. Validated for WB.
Application: WB
Reactivity: Hu, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HLA-DRA1 | HLA-DRA | MHC cell surface glycoprotein | MHC class II antigen DRA | MLRW
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P01903
NCBI GenBank: NM_019111 | NP_061984.2
SwissProt: P01903

Target

Target: Human HLA-DRA
Antigen Species: Human
Gene Name: HLA-DRA

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-216 of human HLA-DRA (NP_061984.2). IKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTE
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)