LSBio
HLA-B27 Antibody (clone HLA-ABC-m3, FITC)

HLA-B27 Antibody (clone HLA-ABC-m3, FITC)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry
Reactivity:Human
Format:Liquid
See all Human HLA-B27 antibodies →
$617.00each
Catalog Number: LS-C57224-1

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Overview

Description
HLA-B27 antibody LS-C57224 is an FITC-conjugated mouse monoclonal antibody to human HLA-B27. Validated for Flow.
Application
FC
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: FC
1:1

Target

Target
Human HLA-B27
Antigen Species
Human
Epitope
Recognizes the HLA-B27 alloantigen. Reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. Affinity studies by Scatchard analysis showed that this has a higher affinity for HLA-B27 (9.7 x 108 M-1) than for HLA B7 (9.5 x 107 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
HLA-ABC-m3
Isotype
IgG2a

Immunogen

Immunogen
Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Liquid
Formulation
PBS, 0.1% Sodium Azide, 0.2% BSA
Concentration
50 µg/mL
Volume
1 Milliliter
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.