LSBio
HLA-A/B/C Antibody (clone MEM-147)

Western blot of human Ramos B cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained byµmAbµMEM-147 andµgoat anti-mouse IgG (H+L)-HRP conjugate.

HLA-A/B/C Antibody (clone MEM-147)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human HLA-A/B/C antibodies →
$316.00each
Catalog Number: LS-C45854-0.1

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Overview

Description
C antibody LS-C45854 is an unconjugated mouse monoclonal antibody to human C (HLA-A / B). Validated for Flow, IP and WB.
Application
FC, IHC, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies

Target

Target
Human HLA-A/B/C
Antigen Species
Human
Epitope
The antibody MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
MEM-147
Isotype
IgG1

Immunogen

Immunogen
PHA-activated peripheral blood lymphocytes
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 15 mM Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C. Do not freeze.
Recommended Storage Buffer
PBS

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.