LSBio
Histone H1 Antibody (clone r1415-1)

Confocal Immunofluorescence image of HeLa cells using Histone H1 Mouse Monoclonal Antibody (r1415-1) Green (CF488) and Phalloidin (Red) is used to label the membranes.

Histone H1 Antibody (clone r1415-1)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Recombinant Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry, Paraffin
Reactivity:Human
Format:Liquid
See all Human Histone H1 antibodies →
$379.00each
Catalog Number: LS-C751168-20

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Overview

Description
Histone H1 antibody LS-C751168 is an unconjugated mouse recombinant monoclonal antibody to human Histone H1. Validated for Flow, IF and IHC.
Application
FC, IF, IHC-P
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Recombinant Antibodies
Recommended Dilution: FC
1 - 2 µg/10E6 cells
Recommended Dilution: IF/ICC
0.5 - 1 µg/ml
Recommended Dilution: IHC-P
1 - 2 µg/ml

Target

Target
Human Histone H1
Antigen Species
Human
Epitope
Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Antibody

Host Species
Mouse
Clonality
Recombinant Monoclonal
Clone
r1415-1
Isotype
IgG2a
Light Chain
Kappa Light Chain

Immunogen

Immunogen
Recombinant full-length human Histone H1 protein. Antigen Molecular Weight: ~30 kDa
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
1 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Concentration
200 µg/mL
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 2 years.
Recommended Storage Buffer
1 mM PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.