LSBio
HIST3H3 Antibody (Methyl-Lys27)

Western blot analysis of extracts of HeLa cells using H3K27me3 antibody. The lane on the left is treated with the antigen-specific peptide.

HIST3H3 Antibody (Methyl-Lys27)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Chromatin Immunoprecipitation, Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human HIST3H3 antibodies →
$288.00each
Catalog Number: LS-C806423-100

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Overview

Description
HIST3H3 antibody LS-C806423 is an unconjugated rabbit polyclonal antibody to HIST3H3 (Methyl-Lys27) from human. It is reactive with human, mouse and rat. Validated for ChrIP, IF, IHC, IP, Peptide-ELISA and WB.
Application
ChIP, ELISA, IF, IHC, IP, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HIST3H3 | Histone H3.1t | H3t | Histone 3, H3 | Histone cluster 3, H3
Recommended Dilution: IF/ICC
1:50 - 1:200
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: IP
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
Q16695
SwissProt
Q16695

Target

Target
Human HIST3H3
Antigen Species
Human
Epitope
Histone H3K27me3 Antibody detects endogenous levels of total Histone H3K27me3.
Gene Name
H3-4

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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