Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Dot Blot of rabbit Histone H3 K4-Me3/K9-Ac Antibody. Lane 1: K4/K9 unmodified. Lane 2: K4-Me3/K9-Ac. Lane 3: K4-Me1. Lane 4: K4-Me2. Lane 5: K4-Me3. Lane 6: K9-Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 K4-Me3/K9-Ac (RABBIT) antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.
HIST3H3 Antibody (Me3-Lys4/Ac-Lys9)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Dot Blot, Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745486-50
Catalog Number: LS-C745486
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- HIST3H3 antibody LS-C745486 is an unconjugated rabbit polyclonal antibody to HIST3H3 (Me3-Lys4/Ac-Lys9) from human. It is reactive with human, mouse, rat and other species. Validated for DB, IF and WB.
- Application
- DB, IF, WB
- Reactivity
- Hu
- Predicted Reactivity
- Helminth / Nematode, Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HIST3H3 | Histone H3.1t | H3t | Histone 3, H3 | Histone cluster 3, H3
- Recommended Dilution: IF/ICC
- 1:10
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q16695
- NCBI GenBank
- NM_003493 | NP_003484.1
- SwissProt
- Q16695
Target
- Target
- Human HIST3H3
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 K4-Me3/K9-Ac from other sources has not been determined.
- Gene Name
- H3-4
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Histone H3 K4-Me3/K9-Ac affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide with a methylation surrounding Lysine 4 and an acetylation surrounding Lysine 9 of human Histone H3.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide, 30% Glycerol
- Concentration
- 0.46 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
