Immunofluorescence staining of H3A in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human H3A polyclonal antibody (dilution ratio 1:1000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.

HIST1H3A Antibody (Preservative Free)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$309.00each

Catalog Number: LS-C818052-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: HIST1H3A antibody LS-C818052 is an unconjugated rabbit polyclonal antibody to human HIST1H3A. Validated for ELISA, ICC, IF, IHC, IP and WB.
Application: ELISA, ICC, IF, IHC-P, IP, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:5000 - 1:10000
Recommended Dilution: IF/ICC: 1:300 - 1:10000
Recommended Dilution: IHC-P: 1:500 - 1:2000
Recommended Dilution: WB: 1:500 - 1:2000
NCBI GenBank: NP_003520.1

Target

Target: Human HIST1H3A
Antigen Species: Human
Epitope: Human HIST1H3A

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant Human HIST1H3A protein
Immunogen Type: Recombinant Immunogen

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.2 µm filtered solution in PBS
Volume: 100 Microliter
Preservative: No
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS