Immunofluorescence staining of HIF1A in HeLa(500uM CoCl for 24hr) cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HIF1A polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
HIF1A / HIF1 Alpha Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C816501-100
Catalog Number: LS-C816501
For bulk orders or custom formulations:
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- Description
- HIF1 Alpha antibody LS-C816501 is an unconjugated rabbit polyclonal antibody to human HIF1 Alpha (HIF1A). Validated for ICC and IF.
- Application
- ICC, IF
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | HIF1A | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- UniProt Number
- Q16665
- NCBI GenBank
- NM_001530 | NP_001521.1
- SwissProt
- Q16665
Target
- Target
- Human HIF1A / HIF1 Alpha
- Antigen Species
- Human
- Epitope
- Human HIF-1 alpha
- Gene Name
- HIF1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- E. coli-derived Human HIF-1 alpha/HIF1A fragment
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.0, 0.03% Proclin 300
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS