Anti-HIF-1alphaHYDROXYP564 Antibody - Western Blot. Western blot of anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kD in size corresponding to HIF1-????The primary antibody was used at a 1:1000 dilution in 2% BLOTTO. Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.
HIF1A / HIF1 Alpha Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C59282-100
Catalog Number: LS-C59282
For bulk orders or custom formulations:
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- Description
- HIF1 Alpha antibody LS-C59282 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Dog, Mouse, Non-Human Primate, Rat, Xenopus
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | HIF1A | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
- Recommended Dilution: ELISA
- 1:5000 - 1:20000
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q16665
- NCBI GenBank
- NM_001530 | NP_001521.1
- SwissProt
- Q16665
Target
- Target
- Human HIF1A / HIF1 Alpha
- Antigen Species
- Human
- Epitope
- This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. A BLAST analysis was used to suggest cross-reactivity with HIF-1a from human, monkey, mouse, rat, dog, bovine and Xenopus sources based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Gene Name
- HIF1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1a.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 65 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at 4°C.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl