Detection of Human HIF1 alpha by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells treated with cobalt chloride-200 mcM (left) or mock treatment (right). Antibody: Affinity purified rabbit anti-HIF1 alpha used at a dilution of 1:100 (2 ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated.
HIF1A / HIF1 Alpha Antibody (aa775-C-term)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287203-2
Catalog Number: LS-C287203
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- Description
- HIF1 Alpha antibody LS-C287203 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa775-C-term) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC, IP and WB. Cited in 1 publication.
- Application
- ICC, IF, IHC, IP, WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Bovine, Chicken, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | HIF1A | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
- Recommended Dilution: IF/ICC
- 1:50 - 1:200
- Recommended Dilution: IHC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- Q16665
- NCBI GenBank
- NM_001530 | NP_001521.1
- SwissProt
- Q16665
Target
- Target
- Human HIF1A / HIF1 Alpha
- Antigen Species
- Human
- Epitope
- Region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1, alpha subunit using the numbering given in Swiss-Prot entry Q16665 (GeneID 3091).
- Gene Name
- HIF1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 3091 corresponding to amino acids between 775 and the C-Terminus of UniprotID: Q16665
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- TBS, 0.09% Sodium Azide, 0.1% BSA
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- TBS
Quantum Blue Reduces the Severity of Woody Breast Myopathy via Modulation of Oxygen Homeostasis-Related Genes in Broiler Chickens. Greene E, Flees J, Dadgar S, Mallmann B, Orlowski S, Dhamad A, Rochell S, Kidd M, Laurendon C, Whitfield H, Brearley C, Rajaram N, Walk C, Dridi S. Frontiers in physiology. 2019 October;10. PubMed: 31632293 PMC: PMC6781743