Anti-KSHV ORF57 Antibody - Western Blot. Western blot of affinity purified anti-KSHV ORF57 to detect KSHV ORF57 in HEK293 cells transfected with ORF57 expression vector and ORF57 truncations, or in KSHV infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow). The membrane was probed with the primary antibody diluted 1:7500 (left) and 1:1000 (right). Personal Communication, V. Majerciak, M. Zheng, CCR-NCI, Bethesda, MD.

HHV-8 ORF57 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Virus / Viral Antigen
Format:	Liquid

See all Human Herpesvirus 8 HHV-8 ORF57 antibodies â†’

$579.00each

Catalog Number: LS-C60137-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: HHV-8 ORF57 antibody LS-C60137 is an unconjugated rabbit polyclonal antibody to HHV-8 ORF57 (C-Terminus) from human herpesvirus 8. It is reactive with human and human herpesvirus 8. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application: ELISA, IHC, WB
Reactivity: Hu, Vir
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:10000 - 1:70000
Recommended Dilution: WB: 1:5000 - 1:8000

Target

Target: Human Herpesvirus 8 HHV-8 ORF57
Antigen Species: Virus / Viral Antigen
Epitope: A BLAST analysis was used to suggest cross-reactivity with ORF57 protein from human herpesvirus 8 types P and M sources based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is unknown.

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1.55 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl

Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral latency. Mariggiò G, Koch S, Zhang G, Weidner-Glunde M, Rückert J, Kati S, Santag S, Schulz TF. PLoS pathogens. 2017;13:e1006335. PubMed: 28430817 PMC: PMC5415203

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