Immunofluorescence staining of HGF in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HGF polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
HGF / Hepatocyte Growth Factor Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818049-100
Catalog Number: LS-C818049
For bulk orders or custom formulations:
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- Description
- Hepatocyte Growth Factor antibody LS-C818049 is an unconjugated rabbit polyclonal antibody to human Hepatocyte Growth Factor (HGF). Validated for ELISA, ICC, IF and IHC.
- Application
- ELISA, ICC, IF, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DFNB39 | Hepatocyte growth factor | Hepatopoeitin-A | HGF | Hepapoietin A | Hepatopoietin-A | Scatter factor) | SF | F-TCF | HGFB | HPTA | Scatter factor
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC-P
- 1:100 - 1:500
- UniProt Number
- P14210
- NCBI GenBank
- CAA34387.1 | X16323
- SwissProt
- P14210
Target
- Target
- Human HGF / Hepatocyte Growth Factor
- Antigen Species
- Human
- Epitope
- Human HGF
- Gene Name
- HGF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant human HGF protein.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS