Hepatitis B x Antigen (HBxAg) Antibody (clone 3F6-G10)

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Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity:	Virus / Viral Antigen
Format:	Liquid

See all Virus Hepatitis B x Antigen (HBxAg) antibodies →

$415.00each

Catalog Number: LS-C57338-0.25

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Quantity

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Overview

Description: Hepatitis B x Antigen (HBxAg) antibody LS-C57338 is an unconjugated mouse monoclonal antibody to virus Hepatitis B x Antigen (HBxAg). Validated for ELISA and IHC.
Application: ELISA, IHC-F, IHC-P
Reactivity: Vir
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: IHC-F: 1:100
Recommended Dilution: IHC-P: 1:100

Target

Target: Virus Hepatitis B x Antigen (HBxAg)
Antigen Species: Virus / Viral Antigen
Epitope: recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kD multifunctional protein involved in the development of liver cirrhosis and hepatocellular carcinoma. Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct. Has been used successfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins. Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera. Fine epitope mapping by phage library screening indicates that the epitope recognized bylies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA.

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 3F6-G10
Isotype: IgG2a

Immunogen

Immunogen: HB-Xag-Protein A Fusion protein.
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.09% Sodium Azide, 0.1% BSA
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.