Immunofluorescence staining of HA tag Antibody, mAb, Rabbit with HA-tagged red fluorescent protein expression in CHO cells. The cells were fixed with 4% Poly-Formaldehyde for 5min, permeabilized with 0.1% TritonX-100 for 5 minutes, and blocked in 3% BSA for 30min at room temperature. The cells were then stained with 1/1000 Rabbit Anti-HA-tag mAb at room temperature for 2h, followed by a further incubation at 37 °C for 1h with Mouse Anti-Rabbit IgG Antibody [iFluor488], mAb at 5 µg/ml.
Hemagglutinin / HA Tag Antibody (clone 109B2)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Format: | Lyophilized |
$393.00each
Catalog Number: LS-C829063-40
Catalog Number: LS-C829063
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- HA Tag antibody LS-C829063 is an unconjugated rabbit monoclonal antibody to HA Tag (Hemagglutinin). Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 0.05 - 0.2 ug/ml
Target
- Target
- Hemagglutinin / HA Tag
- Epitope
- The product recognizes HA tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.
Antibody
- Host Species
- Rb
- Clonality
- Monoclonal
- Clone
- 109B2
- Isotype
- IgG
Immunogen
- Immunogen
- A synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). Conjugated to KLH.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Lyophilized
- Formulation
- Lyopilized from PBS, pH 7.4, 0.02% Sodium Azide
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Store lyophilized at -20°C for up to 1 year. Store reconstituted at 2°C to 8°C for up to 3 weeks. Aliquot and store at -20°C or below for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS