Western Blot of Rabbit anti-HDAC7 antibody. Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4 degrees C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: ~103kDa/ ~103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms ~70 and 40kDa.

HDAC7 Antibody (N-Terminus)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse, Non-Human Primate, Rat
Format:	Liquid

$579.00each

Catalog Number: LS-C209354-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: HDAC7 antibody LS-C209354 is an unconjugated rabbit polyclonal antibody to HDAC7 (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Application: ELISA, IF, IHC, WB
Reactivity: Hu, Ms, NHP, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: DKFZP586J0917 | HD7 | Histone deacetylase 7 | HDAC7 | Histone deacetylase 7A | HD7A | HDAC7A
Recommended Dilution: ELISA: 1:20000 - 1:60000
Recommended Dilution: IF/ICC: 1:100 - 1:500
Recommended Dilution: IHC: 1:100 - 1:500
NCBI GenBank: NM_016596

Target

Target: Human HDAC7
Antigen Species: Human
Epitope: This antibody is specific towards HDAC7. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat and Primate based on 100% sequence homology. Cross-reactivity with HDAC7 from other sources has not been determined.

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: HDAC7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1.16 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl