Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of Rabbit Anti-HDAC1 antibody. Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 5 ug per lane. Primary antibody: HDAC1 antibody at 1ug/mL for overnight at 4 degrees C. Secondary antibody: IRDye800 alpha rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 62 kDa for HDAC1.
HDAC1 Antibody (Internal)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Non-Human Primate, Rat |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C209348-100
Catalog Number: LS-C209348
For bulk orders or custom formulations:
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- Description
- HDAC1 antibody LS-C209348 is an unconjugated rabbit polyclonal antibody to HDAC1 (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, WB
- Reactivity
- Hu, Ms, NHP, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HDAC1 | Histone deacetylase 1 | GON-10 | HD1 | RPD3 | RPD3L1
- Recommended Dilution: ELISA
- 1:20000 - 1:60000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC
- 1:100 - 1:500
- UniProt Number
- Q13547
- NCBI GenBank
- NM_004964 | NP_004955.2
- SwissProt
- Q13547
Target
- Target
- Human HDAC1
- Antigen Species
- Human
- Epitope
- This antibody is specific towards HDAC1. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, and Primate based on 100% sequence homology. Cross-reactivity with HDAC1 from other sources has not been determined.
- Gene Name
- HDAC1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- HDAC1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human HDAC1.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 50% Glycerol
- Concentration
- 0.59 mg/mL
- Preservative
- No
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
