Fluorescent image of HeLa cell stained with HDAC1 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HDAC1 immunoreactivity is localized to Nucleus significantly.

HDAC1 Antibody (aa449-482)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunofluorescence, Western Blot
Reactivity:	Human
Format:	Liquid

$401.00each

Catalog Number: LS-C96644-400

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: HDAC1 antibody LS-C96644 is an unconjugated rabbit polyclonal antibody to human HDAC1 (aa449-482). Validated for IF and WB.
Application: IF, WB
Reactivity: Hu
Predicted Reactivity: Mouse
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HDAC1 | Histone deacetylase 1 | GON-10 | HD1 | RPD3 | RPD3L1
Recommended Dilution: IF/ICC: 1:10 - 1:50
Recommended Dilution: WB: 1:1000
UniProt Number: Q13547
NCBI GenBank: NM_004964 | NP_004955.2
SwissProt: Q13547

Target

Target: Human HDAC1
Antigen Species: Human
Epitope: This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 449-482 amino acids from the C-terminal region of human HDAC1.
Gene Name: HDAC1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Volume: 400 Microliter
Preservative: Yes
Purification Method: Ammonium Sulfate Precipitation

Storage & Handling

Storage Conditions: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer: PBS