Western Blot of Mouse Anti-Hemoglobin beta A-2 Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Lane 7: BSA alone. Load: 50ng per lane. Primary antibody: Anti-HbA-2 antibody at 1µg/mL overnight at 4°C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-073 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 3 specific to HbA-2 only.
HBD / Hemoglobin Delta Antibody (N-Terminus, clone 21G1.F1.B9.G9.D11)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C744871-100
Catalog Number: LS-C744871
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- Description
- Hemoglobin Delta antibody LS-C744871 is an unconjugated mouse monoclonal antibody to human Hemoglobin Delta (HBD) (N-Terminus). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- Delta-globin chain | Delta globin | Delta-globin | Hemoglobin subunit delta | Hemoglobin, delta | HBD | Hemoglobin delta chain
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- P02042
- SwissProt
- P02042
Target
- Target
- Human HBD / Hemoglobin Delta
- Antigen Species
- Human
- Gene Name
- HBD
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 21G1.F1.B9.G9.D11
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Hemoglobin beta A-2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-Terminus of Hb d-subunit conjugated to KLH.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl