Western Blot of Mouse Anti-Hemoglobin A (beta chain) Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Load: 50ng per lane. Primary antibody: Anti-HbA antibody at 1µg/mL overnight at 4°C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-073 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 2 specific to HbA and cross reactivity to HbA-2 seen in Lane 3.
HBB / Hemoglobin Beta Antibody (N-Terminus, clone 14G2.G11.F11)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C744864-100
Catalog Number: LS-C744864
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- Description
- Hemoglobin Beta antibody LS-C744864 is an unconjugated mouse monoclonal antibody to human Hemoglobin Beta (HBB) (N-Terminus). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD113t-C | Hemoglobin beta | HBB | Hemoglobin beta chain | Hemoglobin subunit beta | Beta globin chain | Beta-globin | Hemoglobin, beta
- Recommended Dilution: ELISA
- 1:20000
- Recommended Dilution: WB
- 1 µg/ml
- UniProt Number
- P68871
- NCBI GenBank
- NM_000518, NP_000509.1
- SwissProt
- P68871
Target
- Target
- Human HBB / Hemoglobin Beta
- Antigen Species
- Hu
- Gene Name
- HBB
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 14G2.G11.F11
- Isotype
- IgG2a
- Light Chain
- λ
- Origin Species
- Hu
Immunogen
- Immunogen
- Anti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-Terminus of Hb β-subunit conjugated to KLH.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot A
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl