Western blot analysis of extracts of various cell lines, using HAVCR2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 15s.
HAVCR2 / TIM-3 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C748494-20
Catalog Number: LS-C748494
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- Description
- TIM-3 antibody LS-C748494 is an unconjugated rabbit polyclonal antibody to TIM-3 (HAVCR2) from human. It is reactive with human, mouse and rat. Validated for WB. Cited in 1 publication.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HAVcr-2 | KIM-3 | Kidney injury molecule-3 | T cell immunoglobulin mucin 3 | Tim-3 | TIMD3 | T-cell membrane protein 3 | TIM3 | TIMD-3 | HAVCR2 | T cell immunoglobulin mucin-3 | CD366
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q8TDQ0
- NCBI GenBank
- NM_032782 | NP_116171.3
- SwissProt
- Q8TDQ0
Target
- Target
- Human HAVCR2 / TIM-3
- Antigen Species
- Human
- Gene Name
- HAVCR2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 22-202 of human HAVCR2 (NP_116171.3). SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIRIG
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Identification and characterization of M6903, an antagonistic anti-TIM-3 monoclonal antibody. Dong Zhang, Feng Jiang, Rinat Zaynagetdinov, Hui Huang, Vanita D Sood, Hong Wang, Xinyan Zhao, Molly H Jenkins, Qingyong Ji, Youbin Wang, David P Nannemann, Djordje Musil, John Wesolowski, Andrea Paoletti, Tin Bartholomew, Melissa G Derner, Qi An, Christel Iffland, Joern-Peter Halle. Oncoimmunology. 2020 Apr;9:1744921. PubMed: 32313722 PMC: PMC7153820