Immunofluorescence staining of hela with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
H2AFX / H2AX Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$304.00each
Catalog Number: LS-C670395-50
Catalog Number: LS-C670395
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- H2AX antibody LS-C670395 is an unconjugated rabbit polyclonal antibody to human H2AX (H2AFX). Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
- UniProt Number
- P16104
- NCBI GenBank
- NM_002105 | NP_002096.1
- SwissProt
- P16104
Target
- Target
- Human H2AFX / H2AX
- Antigen Species
- Human
- Gene Name
- H2AX
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Peptide sequence around site of Ser (139) derived from human Histone H2AX.
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
- Recommended Storage Buffer
- 0.01 M PBS