Fluorescent image of HeLa cell stained with GTF2I Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). GTF2I immunoreactivity is localized to Nucleus significantly.
GTF2I / TFII I Antibody (aa281-310)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C159799-400
Catalog Number: LS-C159799
For bulk orders or custom formulations:
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- Description
- TFII I antibody LS-C159799 is an unconjugated rabbit polyclonal antibody to human TFII I (GTF2I) (aa281-310). Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BAP-135 | BAP135 | BTK-associated protein, 135kD | BTK-associated protein 135 | DIWS | IB291 | GTF2I | SRF-Phox1-interacting protein | WBSCR6 | TFII I | TFII-I | BTKAP1 | WBS | GTFII-I | SPIN
- Recommended Dilution: IF/ICC
- 1:10 - 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P78347
- NCBI GenBank
- NM_032999 | NP_127492.1
- SwissProt
- P78347
Target
- Target
- Human GTF2I / TFII I
- Antigen Species
- Human
- Epitope
- This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the N-terminal region of human GTF2I.
- Gene Name
- GTF2I
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS