Immunofluorescence staining of GSK3B in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse GSK3B polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
GSK3B / GSK3 Beta Antibody (Preservative Free)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Mouse |
| Format: | Liquid |
$309.00each
Catalog Number: LS-C818549-100
Catalog Number: LS-C818549
For bulk orders or custom formulations:
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- Description
- GSK3 Beta antibody LS-C818549 is an unconjugated rabbit polyclonal antibody to mouse GSK3 Beta (GSK3B). Validated for ELISA, ICC, IF and IHC.
- Application
- ELISA, ICC, IF, IHC-P
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GSK3B | GSK-3 beta | GSK3 beta | GSK3beta | GSK3beta isoform | TPKI | GSK 3beta | Tau protein kinase I | ZGSK-3beta
- Recommended Dilution: ELISA
- 1:5000 - 1:10000
- Recommended Dilution: IF/ICC
- 1:100 - 1:500
- Recommended Dilution: IHC-P
- 1:100 - 1:500
- UniProt Number
- P49841
- NCBI GenBank
- NM_002093 | NP_002084.2
- SwissProt
- P49841
Target
- Target
- Mouse GSK3B / GSK3 Beta
- Antigen Species
- Mouse
- Epitope
- Mouse GSK3B
- Gene Name
- GSK3B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant Mouse GSK3B Protein
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.2 µm filtered solution in PBS
- Volume
- 100 Microliter
- Preservative
- No
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS