Western blot analysis of extracts of SH-SY5Y cells, using GRHL2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

GRHL2 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Western Blot
Reactivity:	Human
Format:	Liquid

$281.00each

Catalog Number: LS-C496865-20

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Overview

Description: GRHL2 antibody LS-C496865 is an unconjugated rabbit polyclonal antibody to human GRHL2. Validated for WB.
Application: WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: DFNA28 | GRHL2 | BOM | Grainyhead-like 2 (Drosophila) | TFCP2L3
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: Q6ISB3
NCBI GenBank: NM_024915 | NP_079191.2
SwissProt: Q6ISB3

Target

Target: Human GRHL2
Antigen Species: Human
Gene Name: GRHL2

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-250 of human GRHL2 (NP_079191.2). LSVSKASDSQEDQEKRNCLGTSEAQSNLSGGENRVQVLKTVPVNLSLNQDHLENSKREQYSISFPESSAIIPVSGITVVKAEDFTPVFMAPPVHYPRGDGEEQRVVIFEQTQYDVPSLATHSAYLKDDQRSTPDSTYSESFKDAATEKFRSASVGAEEYMYDQTSSGTFQY
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)