Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~100 kDa LGR5 protein using Rabbit Anti-LGR5 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-LGR5 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~100 kDa. Other Band(s): 97 kDa, 70 kDa.
GPR49 / LGR5 Antibody (Atto 594)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$489.00each
Catalog Number: LS-C775219-100
Catalog Number: LS-C775219
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- Description
- LGR5 antibody LS-C775219 is an Atto 594-conjugated rabbit polyclonal antibody to human LGR5 (GPR49). Validated for WB.
- Application
- WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- FEX | G-protein coupled receptor 49 | G protein-coupled receptor 49 | GPR67 | GRP49 | GPR49 | LGR5 | HG38 | G-protein coupled receptor 67
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O75473
- NCBI GenBank
- NM_003667 | NP_003658.1
- SwissProt
- O75473
Target
- Target
- Human GPR49 / LGR5
- Antigen Species
- Human
- Epitope
- Detects 97 kDa. Band at ~97 kDa in isoform 2. Band at ~70 kDa is a degradation product.
- Gene Name
- LGR5
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Synthetic peptide from the mid-protein of Human LGR5 (aa. 500-600)
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Atto 594
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS