Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

GPR135 Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:	Human, Mouse, Rat
Format:	Liquid

$288.00each

Catalog Number: LS-C805739-100

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Overview

Description: GPR135 antibody LS-C805739 is an unconjugated rabbit polyclonal antibody to GPR135 from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Application: ELISA, IF, WB
Reactivity: Hu, Ms, Rt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: G protein-coupled receptor 135 | GPR135 | HUMNPIIY20 | PAFR
Recommended Dilution: IF/ICC: 1:100 - 1:500
Recommended Dilution: WB: 1:500 - 1:1000
UniProt Number: Q8IZ08
NCBI GenBank: NM_022571 | NP_072093.2
SwissProt: Q8IZ08