The Recombinant Human GPC4 (50ng) and Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPC4 anibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human GPC4 Lane 2. : Ramos cell lysate
GPC4 / Glypican 4 Antibody (clone AT51E3)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$310.00each
Catalog Number: LS-C755470-50
Catalog Number: LS-C755470
For bulk orders or custom formulations:
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- Description
- Glypican 4 antibody LS-C755470 is an unconjugated mouse monoclonal antibody to human Glypican 4 (GPC4). Validated for ELISA, Flow, ICC and WB.
- Application
- ELISA, FC, ICC, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- DJ900E8.1 (glypican 4) | Glypican 4 | Glypican proteoglycan 4 | Glypican-4 | GPC4 | K-glypican
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O75487
- NCBI GenBank
- NM_001448 | NP_001439.2
- SwissProt
- O75487
Target
- Target
- Human GPC4 / Glypican 4
- Antigen Species
- Human
- Gene Name
- GPC4
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- AT51E3
- Isotype
- IgG2a
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant human GPC4 (401-529aa) purified from E. coli
- Immunogen Type
- Recombinant Protein Fragment
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
- Concentration
- 1 mg/mL
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS