Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Glypican-3 Mouse Recombinant Monoclonal Ab (rGPC3/863).
GPC3 / Glypican 3 Antibody (clone rGPC3/863, Azide-free, Carrier-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Recombinant Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry, Paraffin |
| Reactivity: | Human |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C751161-100
Catalog Number: LS-C751161
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- Description
- Glypican 3 antibody LS-C751161 is an unconjugated mouse recombinant monoclonal antibody to human Glypican 3 (GPC3). Validated for Flow, IF and IHC.
- Application
- FC, IF, IHC-P
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Recombinant Antibodies
- Alternative Names
- DGSX | Glypican proteoglycan 3 | GPC3 | Glypican 3 | Glypican-3 | GTR2-2 | Intestinal protein OCI-5 | SGB | SGBS1 | SGBS | MXR7 | OCI-5 | OCI5 | SDYS | Secreted glypican-3 | Heparan sulphate proteoglycan
- Recommended Dilution: FC
- 0.5 - 1 µg/10E6 cells
- Recommended Dilution: IF/ICC
- 1 - 2 µg/ml
- Recommended Dilution: IHC-P
- 1 - 2 µg/ml
- UniProt Number
- P51654
- NCBI GenBank
- NM_004484 | NP_004475.1
- SwissProt
- P51654
Target
- Target
- Human GPC3 / Glypican 3
- Antigen Species
- Human
- Epitope
- Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm's tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
- Gene Name
- GPC3
Antibody
- Host Species
- Mouse
- Clonality
- Recombinant Monoclonal
- Clone
- rGPC3/863
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
- Origin Species
- Human
Immunogen
- Immunogen
- Recombinant full-length human GPC3 protein. Antigen Molecular Weight: 67 kDa
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 1 mM PBS
- Concentration
- 1 mg/mL
- Preservative
- No
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C to -80°C for up to 2 years.
- Recommended Storage Buffer
- 1 mM PBS