Immunohistochemistry of rabbit anti-Glypican antibody. Tissue: skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Glypican antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Glypican as precipitated red signal with hematoxylin purple nuclear counterstain.
GPC1 / Glypican Antibody (Internal)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C744975-25
Catalog Number: LS-C744975
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- Description
- Glypican antibody LS-C744975 is an unconjugated rabbit polyclonal antibody to Glypican (GPC1) (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Glypican 1 | Glypican | Glypican-1 | Glypican proteoglycan 1 | GPC1
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: IHC
- 10 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P35052
- NCBI GenBank
- NM_002081 | NP_002072.2
- SwissProt
- P35052
Target
- Target
- Human GPC1 / Glypican
- Antigen Species
- Human
- Gene Name
- GPC1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Glypican-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl