Anti-Rat IgG Secondary Antibody
Catalog Number: LS-C828036
3 sizes / from $323.00
Antibody: Rat Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Rat
Format: Liquid
ELISA results of purified Goat F(ab')2 Anti-Rat IgG F(c) Biotin Conjugated Antibody (Min X Bv Hs & Hu serums) tested against purified Rat IgG F(c). Each well was coated in duplicate with 1.0 µg of Rat IgG F(c) (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate, and TMB-1000 substrate.
Goat Anti-Rat IgG Fc Secondary Antibody (Biotin)
Secondary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Western Blot |
| Reactivity: | Rat |
| Format: | Lyophilized |
$384.00each
Catalog Number: LS-C61512-500
Catalog Number: LS-C61512
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- Description
- Rat IgG Fc antibody LS-C61512 is a biotin-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
- Application
- ELISA, IB, IHC, WB
- Reactivity
- Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: ELISA
- 1:20000 - 1:100000
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
Target
- Target
- Rat IgG Fc
- Antigen Species
- Rat
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab) or Bovine, Horse and Human Serum Proteins.
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Rat IgG F(c) fragment.
- Immunogen Type
- Native Protein (Purified from Natural Source)
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl