LSBio
Goat Anti-Rabbit IgG Secondary Antibody (HRP)

RABBIT IgG (H&L) Antibody Peroxidase Conjugated Western Blot. Western blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 ug per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kD, 42 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Goat Anti-Rabbit IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$324.00each
Catalog Number: LS-C60884-2

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Overview

Description
Rabbit IgG antibody LS-C60884 is an HRP-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB. Cited in 2 publications.
Application
DB, ELISA, IHC, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:150000 - 1:250000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:10000 - 1:20000

Target

Target
Rabbit IgG
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Following resuspension aliquot and store at -20°C. Avoid freeze-thaw cycles and dilute immediately before use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Gingival tissue human beta-defensin levels in relation to infection and inflammation. Meltem Özdemir, Feriha Caglayan, Floris J Bikker, Pirkko Pussinen, Eija Könönen, Nermin Yamalik, Mervi Gürsoy, Dareen Fteita, Kamran Nazmi, Güliz N Güncü, Milla Pietiäinen, Mimmi Tolvanen, Ulvi Kahraman Gürsoy. Journal of clinical periodontology. 2020 March;47:309-318.

PubMed: 31799742


Gene expression is stable in a complete CIB1 knockout keratinocyte model. Elias Imahorn, Magomet Aushev, Stefan Herms, Per Hoffmann, Sven Cichon, Julia Reichelt, Peter H Itin, Bettina Burger. Scientific reports. 2020 September;10:14952.

PubMed: 32917957 · PMC: PMC7486891

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