LSBio
Goat Anti-Rabbit IgG Secondary Antibody (FITC)

Western blot of Fluorescein conjugated Goat F(ab')2 Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

Goat Anti-Rabbit IgG Secondary Antibody (FITC)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Flow Cytometry, Immunofluorescence, Other, Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$360.00each
Catalog Number: LS-C61475-500

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Overview

Description
Rabbit IgG antibody LS-C61475 is an FITC-conjugated goat polyclonal antibody. Validated for FLISA, Flow, IF and WB.
Application
FC, IF, Other, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: FC
1:500 - 1:2500
Recommended Dilution: IF/ICC
1:1000 - 1:5000
Recommended Dilution: Other
FLISA: 1:10000 - 1:50000

Target

Target
Rabbit IgG
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted. Protect from light.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.