LSBio
Goat Anti-Rabbit IgG Secondary Antibody (Biotin)

Western blot of Biotin conjugated Goat Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: Biotin conjugated Goat Anti-Rabbit IgG Pre-Adsorbed at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

Goat Anti-Rabbit IgG Secondary Antibody (Biotin)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$396.00each
Catalog Number: LS-C60867-1

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Overview

Description
Rabbit IgG antibody LS-C60867 is a biotin-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB. Cited in 1 publication.
Application
ELISA, IB, IHC, WB
Reactivity
Rb
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:400000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Rabbit IgG
Antigen Species
Rabbit
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Active exercise promotes Achilles tendon healing and is accompanied by the upregulation of collapsin response mediator protein‑2 in rats. Qianman B, Jialihasi A, Asilehan B, Kubai A, Aibek R, Wupuer A, Tangkejie W, Maimaitiaili A, Shawutali N, Badelhan A, Aizezi A, Aisaiding A, Wuerliebieke J, Bakyt Y, Makemutibieke E, Jielile J. Molecular medicine reports. 2017;16:2355-2360. PubMed: 28677754

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