Anti-Mouse IgG Secondary Antibody
Catalog Number: LS-C664099
6 sizes / from $319.00
Antibody: Mouse Monoclonal
Applications: Western Blot
Reactivity: Rabbit
Format: Liquid
Western Blot of ATTO 647N conjugated Goat anti-Mouse IgG3 (gamma 3 chain) Pre-adsorbed secondary antibody. Lane 1: Mouse IgG3. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 647N goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG3. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti-Mouse IgG3 Secondary Antibody (Atto 647N)
Secondary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Other, Western Blot |
| Reactivity: | Mouse |
| Format: | Lyophilized |
$482.00each
Catalog Number: LS-C209483-500
Catalog Number: LS-C209483
For bulk orders or custom formulations:
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- Description
- Mouse IgG3 antibody LS-C209483 is an Atto 647N-conjugated goat polyclonal antibody. Validated for FLISA, Flow, IF and WB. Cited in 2 publications.
- Application
- FC, IF, IHC, Other, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Secondary Antibodies
- Recommended Dilution: FC
- 1:500 - 1:2500
- Recommended Dilution: IF/ICC
- 1:5000
- Recommended Dilution: WB
- 1:4000 - 1:20000
Target
- Target
- Mouse IgG3
- Antigen Species
- Mouse
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Mouse IgG3 heavy chain.
Format
- Conjugate/Label
- Atto 647N
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Tolerogenic properties of liver macrophages in non-alcoholic steatohepatitis. Lorenzo A Orci, Mario Kreutzfeldt, Nicolas Goossens, Laura Rubbia-Brandt, Florence Slits, Karim Hammad, Vaihere Delaune, Graziano Oldani, Francesco Negro, Sophie Clément, Carmen Gonelle-Gispert, Léo H Buhler, Christian Toso, Stéphanie Lacotte. Liver international : official journal of the International Association for the Study of the Liver. 2020 March;40:609-621.
PubMed: 31872499
Neurons under T Cell Attack Coordinate Phagocyte-Mediated Synaptic Stripping. Giovanni Di Liberto, Stanislav Pantelyushin, Mario Kreutzfeldt, Nicolas Page, Stefano Musardo, Roland Coras, Karin Steinbach, Ilena Vincenti, Bogna Klimek, Thomas Lingner, Gabriela Salinas, Nathalie Lin-Marq, Ori Staszewski, Marta Joana Costa Jordão, Ingrid Wagner, Kristof Egervari, Matthias Mack, Camilla Bellone, Ingmar Blümcke, Marco Prinz, Daniel D Pinschewer, Doron Merkler. Cell. 2018 October;175:458-471.e19.
PubMed: 30173917
