LSBio
Goat Anti-Human IgM Secondary Antibody

ELISA results of purified Goat anti-Human IgM antibody tested against purified human IgM, IgG Fab, IgG Fc, IgG Fab2. Each well was coated in duplicate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.

Goat Anti-Human IgM Secondary Antibody

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Other, Western Blot
Reactivity:Human
Format:Liquid
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$300.00each
Catalog Number: LS-C60527-1

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Overview

Description
Human IgM antibody LS-C60527 is an unconjugated goat polyclonal antibody. Validated for ELISA, IE, IHC and WB.
Application
ELISA, IHC, IP, Other, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Human IgM
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against other human heavy or light chain proteins.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Human IgM whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.