LSBio
Goat Anti-Human IgG Secondary Antibody (HRP)

Western blot of Goat anti-Human IgG, IgA, IgM Peroxidase Conjugated Antibody. Lane 1: Human IgG. Lane 2: Human IgA. Lane 3: Human IgM. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: Appropriate banding pattern for Human IgG, IgA, and IgM is shown. Other band(s): Splice variants and isoforms. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Goat Anti-Human IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Other, Western Blot
Reactivity:Human
Format:Lyophilized
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$393.00each
Catalog Number: LS-C60512-2

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Overview

Description
Human IgG antibody LS-C60512 is an HRP-conjugated goat polyclonal antibody. Validated for ELISA, IE, IHC, IP and WB.
Application
DB, ELISA, IHC, IP, Other, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: IP
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Human IgG
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgA and Human IgM. This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Human IgG IgA IgM (H&L) was produced by repeated immunization with Human IgG, IgA and IgM whole molecules in goat.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Horseradish Peroxidase
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
2 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.