LSBio
Goat Anti-Human IgG Fc Secondary Antibody

ELISA results of purified Goat anti-Human IgG F(c) antibody (min x Mouse serum proteins) tested against purified Human IgG F(c) Each well was coated in duplicate with 1.0 µg of Human IgG F(c) as well as Mouse IgG The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) and TMB substrate

Goat Anti-Human IgG Fc Secondary Antibody

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human IgG Fc antibodies →
$308.00each
Catalog Number: LS-C60501-1

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Overview

Description
Human IgG Fc antibody LS-C60501 is an unconjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:15000 - 1:60000
Recommended Dilution: IHC
1:1000 - 1:3000
Recommended Dilution: WB
1:1000 - 1:5000

Target

Target
Human IgG Fc
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab) or Mouse Serum Proteins. Specificity was confirmed by ELISA minimal cross reactivity against Mouse IgG.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Human IgG F(c) fragment.
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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