LSBio
Goat Anti-Human IgG Fab'2 Secondary Antibody (Biotin)

ELISA results of purified Goat F(ab')2 Anti-HUMAN IgG F(ab')2 Biotin Conjugated Antibody (Min X Bv Hs Ms & Rt Serum Proteins) tested against purified HUMAN IgG F(ab')2 . Each well was coated in duplicate with 1.0 µg of HUMAN IgG F(ab')2 (green line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate, and TMB-1000 substrate.

Goat Anti-Human IgG Fab'2 Secondary Antibody (Biotin)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Lyophilized
See all Human IgG Fab'2 antibodies →
$426.00each
Catalog Number: LS-C61385-1

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Overview

Description
Human IgG Fab'2 antibody LS-C61385 is a biotin-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:500000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:5000 - 1:40000

Target

Target
Human IgG Fab'2
Antigen Species
Human
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgG, Human IgG F(ab')2 and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse, and Rat Serum Proteins.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Human IgG F(ab')2 fragment
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C while lyophilized, once reconstituted aliquot and freeze at -20°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.