Dot Blot results of rabbit Anti-Glutamate Dehydrogenase Biotin Conjugated. Antigen: Glutamate Dehydrogenase. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Glutamate Dehydrogenase Biotin 1µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP (S000-03) at 1:40,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.

GLUD1/Glutamate Dehydrogenase Antibody (Liquid, Biotin)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunoprecipitation, Western Blot
Reactivity:	Bovine
Format:	Liquid

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$311.00each

Catalog Number: LS-C745134-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Glutamate Dehydrogenase antibody LS-C745134 is a biotin-conjugated rabbit polyclonal antibody to bovine Glutamate Dehydrogenase (GLUD1). Validated for DB, ELISA, IP and WB.
Application: DB, ELISA, IP, WB
Reactivity: Bov
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: GLUD1 | GDH1 | GDH 1 | Glutamate dehydrogenase 1 | Glutamate dehydrogenase 2 | GDH | GLUD
UniProt Number: P00367
NCBI GenBank: NM_005271 | NP_005262.1
SwissProt: P00367

Target

Target: Bovine GLUD1/Glutamate Dehydrogenase
Antigen Species: Bovine
Epitope: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. Cross reactivity against Glutamate Dehydrogenase from other sources may occur but have not been specifically determined.
Gene Name: GLUD1

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Glutamate Dehydrogenase [Bovine Liver].

Format

Conjugate/Label: Biotin
Format: Liquid
Formulation: 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Ion Exchange Chromatography

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl