Goat anti Glycerol Kinase antibody was used to detect purified Glycerol Kinase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
GK / Glycerol Kinase Antibody (Lyophilized, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Bacteria |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C744763-100
Catalog Number: LS-C744763
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- Description
- Glycerol Kinase antibody LS-C744763 is a biotin-conjugated goat polyclonal antibody to cellulomonas Glycerol Kinase (GK). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Bac
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GKD | GK1 | GK | Glycerokinase | Glycerol kinase
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:500 - 1:2500
- UniProt Number
- P32189
- NCBI GenBank
- NM_000167, NP_000158.1
- SwissProt
- P32189
Target
- Target
- Cellulomonas GK / Glycerol Kinase
- Antigen Species
- Bac
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species]. Cross reactivity against Glycerol Kinase from other sources is unknown.
- Gene Name
- GK
Antibody
- Host Species
- Gt
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Glycerol Kinase [Cellulomonas species].
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- IEX
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl