Western blot analysis of extracts of various cell lines, using GJA1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
GJA1 / CX43 / Connexin 43 Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
$281.00each
Catalog Number: LS-C747120-20
Catalog Number: LS-C747120
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- Description
- Connexin 43 antibody LS-C747120 is an unconjugated rabbit polyclonal antibody to Connexin 43 (GJA1 / CX43) from human. It is reactive with human, mouse and rat. Validated for WB.
- Application
- WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Connexin-43 | DFNB38 | Connexin 43 | Gap junction alpha-1 protein | GJA1 | GJAL | HSS | Gap junction protein alpha 1 | ODDD | ODD | AVSD3 | CX43 | HLHS1 | ODOD | SDTY3
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P17302
- NCBI GenBank
- NM_000165, NP_000156.1
- SwissProt
- P17302
Target
- Target
- Human GJA1 / CX43 / Connexin 43
- Antigen Species
- Hu
- Gene Name
- GJA1
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Hu
Immunogen
- Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 233-382 of human GJA1 (NP_000156.1). FKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI
- Immunogen Type
- Fusion-P
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
- Volume
- 20 Microliter
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS