Anti-GGA1 Antibody - Western Blot. Western blot of Affinity Purified anti-GGA1 antibody shows detection of bands at ~100 kD corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells over- expressing the recombinant protein (arrowhead). Approximately 35 ug of lysate was separated on a 4-20% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred overnight at 4? followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.