Anti-NAG-1/GDF15 (C-terminal) Antibody - Western Blot. Western blot of affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kD band corresponding to monomer and a 28 kD band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. This image was taken for the unconjugated form of this product. Other forms have not been tested.

GDF15 Antibody (Biotin)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Human, Mouse
Format:	Lyophilized

$555.00each

Catalog Number: LS-C154298-100

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: GDF15 antibody LS-C154298 is a biotin-conjugated rabbit polyclonal antibody to GDF15 from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Hu, Ms
Predicted Reactivity: Cynomolgus Monkey, Non-Human Primate
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta
Recommended Dilution: ELISA: 1:20000 - 1:100000
Recommended Dilution: IHC: 1:1000 - 1:5000
Recommended Dilution: WB: 1:2000 - 1:10000
UniProt Number: Q99988
NCBI GenBank: NM_004864 | NP_004855.2
SwissProt: Q99988

Target

Target: Human GDF15
Antigen Species: Human
Epitope: This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Gene Name: GDF15

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Nag-1 Antibody Biotin Conjugated antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Biotin
Format: Lyophilized
Formulation: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store at 4°C prior to resoration. Aliquot and store at -20°C for 12 months. Avoid freeze-thaw cycles. Dilute only prior to immediate use.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl