Immunofluorescence staining of GCLM in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human GCLM polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.

GCLM Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunocytochemistry, Immunofluorescence
Reactivity:	Human
Format:	Liquid

$212.00each

Catalog Number: LS-C816429-50

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: GCLM antibody LS-C816429 is an unconjugated rabbit polyclonal antibody to human GCLM. Validated for ICC and IF.
Application: ICC, IF
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: GCLM | Gamma-ECS regulatory subunit | GLCLR | GCS light chain | GSC light chain
Recommended Dilution: IF/ICC: 1:100 - 1:500
UniProt Number: P48507
NCBI GenBank: NM_002061 | NP_002052.1
SwissProt: P48507

Target

Target: Human GCLM
Antigen Species: Human
Epitope: Human GCLM
Gene Name: GCLM

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: E. coli-derived Human GCLM fragment
Immunogen Type: Recombinant Protein Fragment

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, pH 7.0, 0.03% Proclin 300
Volume: 50 Microliter
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Recommended Storage Buffer: PBS